--- title: [App Note] Limula Automated T Cell Selection on End-to-End LimONE description: Explore Charles River data showing how microbubble T cell isolation enhances CAR T performance, purity, and viability in GMP manufacturing workflows. --- ![Webinar Landing Page Banner-1](https://info.akadeum.com/hs-fs/hubfs/Webinar%20Landing%20Page%20Banner-1.png?width=2000&height=400&name=Webinar%20Landing%20Page%20Banner-1.png "Webinar Landing Page Banner-1") [![Akadeum Life Sciences, Inc](https://info.akadeum.com/hs-fs/hubfs/Akadeum-Logo-Transparent-2.png?width=796&height=797&name=Akadeum-Logo-Transparent-2.png "Akadeum Life Sciences, Inc")](https://www.akadeum.com/) ![app-note-tag](https://info.akadeum.com/hs-fs/hubfs/Akadeum_App-Note-Tag_2x.png?width=100&name=Akadeum_App-Note-Tag_2x.png) ## Automated T Cell Selection and Seeding in Under 2 Hours on the End-to-End LimONE Platform This application note demonstrates how Limula’s automated LimONE platform integrates **T cell enrichment, seeding, and activation into a single streamlined workflow**, reducing Day-0 processing time while maintaining high-quality cell output. Using Akadeum microbubble-based negative selection, the workflow enables rapid enrichment of untouched T cells directly from PBMC input within a closed automated process. -  Automated Day-0 workflow from input to seeded culture -  93% CD3⁺ purity with >68% recovery -  Closed automated processing with minimal hands-on time -  Integrated, scalable workflow compatible with GMP environments Featured Products: [Human T Cell Leukopak Isolation Kit](https://www.akadeum.com/product/microbubble-leukopak-human-t-cell-isolation-kit/) ![App Note Screenshot_Limula LimONE](https://info.akadeum.com/hs-fs/hubfs/App%20Note%20Screenshot_Limula%20LimONE.png?width=438&height=594&name=App%20Note%20Screenshot_Limula%20LimONE.png "App Note Screenshot_Limula LimONE") --- ### Fill out the form to access the application note. 734.707.1233 info@akadeum.com 674 S Wagner Rd Ann Arbor, MI 48103 [![Akadeum Life Sciences](https://info.akadeum.com/hs-fs/hubfs/Akadeum_February2019%20/images/logo-footer.png?width=80&height=85&name=logo-footer.png)](https://www.akadeum.com/) - - - - [Products](https://www.akadeum.com/products/) - [Partnerships](https://www.akadeum.com/partnerships/) - [Solutions](https://www.akadeum.com/technology/) - [Resources](https://www.akadeum.com/resources/) © 2024 [Akadeum Life Sciences](https://www.akadeum.com/)