---
title: [App Note] End-To-End Production of T Cells on the Cocoon® Platform, Using Buoyancy-Activated Cell Sorting™ For T Cell Isolation From Leukopaks
description: View the performance data of SAE and downstream expansion of T Cells on the G Rex Platform
---

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## A Positive Selection Method for Highly Activated, Less Exhausted Cells: Akadeum’s Human T Cell Selection, Activation and Expansion Kit

Isolation and activation of T cells with Akadeum's Human T Cell Selection, Activation, and Expansion Kit can be complemented with expansion of the cells on the Wilson Wolf G-Rex® culture system. In this app note we demonstrate how the combination of the microbubbles and G-Rex® system promotes cell health and expansion while causing less cell exhaustion than magnetics. Explore how cells isolated with bubbles:

- Maintain lower expression of PD-1
- Express low levels of HLA-DR and CD25
- Have consistent cell ratios after expansion
- Are healthier than cells isolated with magnetics

Featured Products: [Human T Cell Selection, Activation, and Expansion Kit (GMP)](https://www.akadeum.com/product/human-t-cell-activation-expansion-kit/?srsltid=AfmBOoomj9NlUVvN-O0bCEdyamTxLp-nwdXro_3olk52FvyXezL1vfzW)

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