--- title: [App Note] Greater Potency Begins with Better Cell Isolation description: Explore Charles River data showing how microbubble T cell isolation enhances CAR T performance, purity, and viability in GMP manufacturing workflows. --- ![Webinar Landing Page Banner-1](https://info.akadeum.com/hs-fs/hubfs/Webinar%20Landing%20Page%20Banner-1.png?width=2000&height=400&name=Webinar%20Landing%20Page%20Banner-1.png "Webinar Landing Page Banner-1") [![Akadeum Life Sciences, Inc](https://info.akadeum.com/hs-fs/hubfs/Akadeum-Logo-Transparent-2.png?width=796&height=797&name=Akadeum-Logo-Transparent-2.png "Akadeum Life Sciences, Inc")](https://www.akadeum.com/) ![app-note-tag](https://info.akadeum.com/hs-fs/hubfs/Akadeum_App-Note-Tag_2x.png?width=100&name=Akadeum_App-Note-Tag_2x.png) ## Greater Potency Begins with Better Cell Isolation: Charles River Laboratories Demonstrates Next-Level Performance with Akadeum's Microbubble Cell Isolation Charles River and Akadeum collaborated in a three-arm study using cryopreserved leukopaks, including an arm incorporating platelet depletion using Fresenius Kabi’s Cue system. The study compared Akadeum’s in-bag microbubble isolation with magnetic methods to evaluate workflow efficiency, cell quality, and downstream CAR T potency. This application note explores how isolation method choice can influence viability, phenotype preservation, and therapeutic performance in CAR T manufacturing. - Higher CAR T killing potential with preserved cell viability - Consistent CD4/CD8 balance and preserved memory phenotypes - Faster isolation time compared to magnetic selection methods - GMP-ready workflow now integrated into Charles River’s Flex Platform Featured Products: [Human T Cell Leukopak Isolation Kit](https://www.akadeum.com/product/microbubble-leukopak-human-t-cell-isolation-kit/) ![Akadeum_App-Note_CRL_Greater_Potency](https://info.akadeum.com/hs-fs/hubfs/Akadeum_App-Note_CRL_Greater_Potency.png?width=154&height=200&name=Akadeum_App-Note_CRL_Greater_Potency.png "Akadeum_App-Note_CRL_Greater_Potency") --- ### Fill out the form to access the application note. 734.707.1233 info@akadeum.com 674 S Wagner Rd Ann Arbor, MI 48103 [![Akadeum Life Sciences](https://info.akadeum.com/hs-fs/hubfs/Akadeum_February2019%20/images/logo-footer.png?width=80&height=85&name=logo-footer.png)](https://www.akadeum.com/) - - - - [Products](https://www.akadeum.com/products/) - [Partnerships](https://www.akadeum.com/partnerships/) - [Solutions](https://www.akadeum.com/technology/) - [Resources](https://www.akadeum.com/resources/) © 2024 [Akadeum Life Sciences](https://www.akadeum.com/)