Greater Potency Begins with Better Cell Isolation: Charles River Laboratories Demonstrates Next-Level Performance with Akadeum's Microbubble Cell Isolation

Charles River and Akadeum collaborated in a three-arm study using cryopreserved leukopaks, including an arm incorporating platelet depletion using Fresenius Kabi’s Cue system. The study compared Akadeum’s in-bag microbubble isolation with magnetic methods to evaluate workflow efficiency, cell quality, and downstream CAR T potency. This application note explores how isolation method choice can influence viability, phenotype preservation, and therapeutic performance in CAR T manufacturing.

• Higher CAR T killing potential with preserved cell viability
• Consistent CD4/CD8 balance and preserved memory phenotypes
• Faster isolation time compared to magnetic selection methods
• GMP-ready workflow now integrated into Charles River’s Flex Platform

Featured Products: Human T Cell Leukopak Isolation Kit