---
title: "[App Note] Healthier Cells Post-Electroporation: Pairing Akadeum’s Human T Cell Leukopak Kit-GMP with MaxCyte’s ExPERT GTx® Instrument"
description: Learn about the importance of a gentle cell isolation and the positive impact it has on the success of electroporation-based gene delivery, post-electroporation viability, and CAR+ cell expansion.
---

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## Healthier Cells Post-Electroporation: Pairing Akadeum’s Human T Cell Leukopak Kit-GMP with MaxCyte’s ExPERT GTx® Instrument

In this app note, we discuss the importance of a gentle cell isolation and the positive impact it has on the success of electroporation-based gene delivery, post-electroporation viability, and CAR+ cell expansion. Data is presented to support microbubbles as the ideal isolation technology to pair with modular workflows due to their consistent, gentle nature, resulting in healthier cells, shorter cost-effective workflows and the ability to scale up without impacting performance or increasing the therapeutic footprint.

Three key takeaways:

1. In-bag microbubble cell isolation preserves T cell health via minimal handling time
2. Healthier cells result in increased cell viability by 10% and therapeutically relevant cell phenotypes with the MaxCyte ExPERT GTx® Instrument, compared to magnetics
3. A shorter, more efficient workflow that minimizes required instrumentation, reduces costs, and removes unnecessary processing steps that can compromise cell health

Featured Products: [Human T Cell Leukopak Isolation Kit - GMP Grade](https://www.akadeum.com/product/microbubble-leukopak-human-t-cell-isolation-kit-gmp/)<https://www.akadeum.com/product/microbubble-leukopak-human-t-cell-isolation-kit/>

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