Healthier Cells Post-Electroporation: Pairing Akadeum’s Human T Cell Leukopak Kit-GMP with MaxCyte’s ExPERT GTx^® Instrument

In this app note, we discuss the importance of a gentle cell isolation and the positive impact it has on the success of electroporation-based gene delivery, post-electroporation viability, and CAR+ cell expansion. Data is presented to support microbubbles as the ideal isolation technology to pair with modular workflows due to their consistent, gentle nature, resulting in healthier cells, shorter cost-effective workflows and the ability to scale up without impacting performance or increasing the therapeutic footprint.

Three key takeaways:

1. In-bag microbubble cell isolation preserves T cell health via minimal handling time

2. Healthier cells result in increased cell viability by 10% and therapeutically relevant cell phenotypes with the MaxCyte ExPERT GTx^® Instrument, compared to magnetics

3. A shorter, more efficient workflow that minimizes required instrumentation, reduces costs, and removes unnecessary processing steps that can compromise cell health

Featured Products: Human T Cell Leukopak Isolation Kit - GMP Grade