Automated T Cell Selection and Seeding in Under 2 Hours on the End-to-End LimONE Platform

This application note demonstrates how Limula’s automated LimONE platform integrates T cell enrichment, seeding, and activation into a single streamlined workflow, reducing Day-0 processing time while maintaining high-quality cell output.

Using Akadeum microbubble-based negative selection, the workflow enables rapid enrichment of untouched T cells directly from PBMC input within a closed automated process.

•  Automated Day-0 workflow from input to seeded culture
•  93% CD3⁺ purity with >68% recovery
•  Closed automated processing with minimal hands-on time
•  Integrated, scalable workflow compatible with GMP environments
  

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