DOWNLOAD OUR RECENT POSTER PRESENTATION:
Nucleic Acid Extraction with Microbubbles from Saliva Enables Enrichment from Larger Sample Volume
Saliva presents an excellent option for non-invasive self-collected samples for screening studies. Previous research suggests using saliva to quantifiably measure analytes is predictive of disease presentation. Still, technical hurdles have prevented efficient and cost-effective scaling that would allow widespread adoption of saliva testing. Akadeum’s functionalized buoyant microbubbles overcome the limitations of magnetics and enable quantitative large-volume saliva testing. In head-to-head comparisons with the MagMAX™ Viral RNA Isolation Kit (ThermoFisher Scientific), Akadeum’s microbubble-based nucleic acid extraction protocol routinely achieved 10-25 times more viral RNA detected by downstream qPCR. This enhancement in sensitivity was shown to be robust and reproducible and translated into greater concordance with NPS tests. Akadeum microbubbles represent the next generation of isolation technology by offering the tools needed to identify patients early in disease, those with low viral load, or positive samples diluted in a pooled saliva setting.
Akadeum's Microbubbles represent the next generation of isolation technology by offering the tools needed to identify patients early in disease, those with low viral load, or positive samples diluted in a pooled saliva application.
Download the poster to learn more:
- Effective novel extraction protocol developed
- Up to 25x more RNA extracted than magnetics
- Specifically optimized for saliva
- Enables full interrogation of large volumes and easily scalable across volumes and protocols
- Sensitive – 50 cp/mL LOD overcomes false negatives and dilution of pooling
- Easily automated – Increased throughput and accurate detection ≤ 140 cp/mL