--- title: "From Leukopak to CAR T Harvest in Seven Days: A Closed, Modular Workflow" description: See how Akadeum, BlueWhale Bio, and ScaleReady built a closed, bead-free CAR T workflow delivering up to 850 million CAR+ cells in seven days. --- ![Webinar Landing Page Banner-1](https://info.akadeum.com/hs-fs/hubfs/Webinar%20Landing%20Page%20Banner-1.png?width=2000&height=400&name=Webinar%20Landing%20Page%20Banner-1.png "Webinar Landing Page Banner-1") [![Akadeum Life Sciences, Inc](https://info.akadeum.com/hs-fs/hubfs/Akadeum-Logo-Transparent-2.png?width=796&height=797&name=Akadeum-Logo-Transparent-2.png "Akadeum Life Sciences, Inc")](https://www.akadeum.com/) ## ![Akadeum\_App-Note-Tag\_2x](https://info.akadeum.com/hs-fs/hubfs/Akadeum_App-Note-Tag_2x.png?width=92&height=29&name=Akadeum_App-Note-Tag_2x.png) Bead-Free CAR T Manufacturing in Seven Days with a Modular, Closed Workflow In this application note, Akadeum Life Sciences, BlueWhale Bio, and ScaleReady demonstrate how their complementary technologies combine into a single modular, functionally closed, bead-free CAR T manufacturing workflow at a clinically relevant scale. Using Akadeum microbubble-based negative selection, untouched T cells were gently enriched in-bag directly from leukopak material, then activated and transduced on Day 0 with BlueWhale Bio’s Synecta™ T1 and expanded in ScaleReady’s G-Rex® 100M-CS through Day 7 harvest. → Over 90% CD3⁺ purity after isolation in both donors → 1.5 to 2.8 billion viable cells and 600 to 850 million CAR⁺ cells by Day 7 from approximately 100 million starting T cells → Activation and transduction consolidated on Day 0 with no bead removal or soluble cytokines → Predominantly stem cell memory and central memory phenotype preserved at harvest **Featured Products:** [Human T Cell Leukopak Isolation Kit](https://www.akadeum.com/product/microbubble-leukopak-human-t-cell-isolation-kit-gmp/) ![Full Logo - Color\_BWB](https://info.akadeum.com/hs-fs/hubfs/Logos/Full%20Logo%20-%20Color_BWB.png?width=2000&height=885&name=Full%20Logo%20-%20Color_BWB.png "Full Logo - Color_BWB") ### Fill out the form to access the application note. 734.707.1233 info@akadeum.com 674 S Wagner Rd Ann Arbor, MI 48103 [![Akadeum Life Sciences](https://info.akadeum.com/hs-fs/hubfs/Akadeum_February2019%20/images/logo-footer.png?width=80&height=85&name=logo-footer.png)](https://www.akadeum.com/) - - - - [Products](https://www.akadeum.com/products/) - [Partnerships](https://www.akadeum.com/partnerships/) - [Solutions](https://www.akadeum.com/technology/) - [Resources](https://www.akadeum.com/resources/) © 2024 [Akadeum Life Sciences](https://www.akadeum.com/)