DOWNLOAD THE APP NOTE:
Exceptionally Gentle Mouse B Cell Enrichment with Microbubbles Maintains Cell Health & Physiology
Analysis of B cell function and regulation requires a highly purified population of unaltered B cells, but traditional separation technologies like magnetic bead-based sorting can be harsh on cells and ultimately produce less-than desirable results.
Buoyancy-Activated Cell Sorting (BACS) microbubble technology from Akadeum is a truly novel, non-invasive method for capturing unaltered cells in their natural physiological state.
Download this app note to see how researchers in laboratories across the country have used Akadeum's Mouse B Cell Isolation Kit to investigate critical aspects of B cell biology, including the distribution of B cell receptors (BCRs), the antigen-presenting capacity of B cells, and the differentiation potential of B cells following enrichment.
- BCR isotype distribution remains unchanged following microbubble-based enrichment
- Microbubble isolation retains antigen presenting capacity of B cells
- B cell differentiation potential is maintained through microbubble-based separation
