All-in-one Automated CAR-T manufacturing using next-generation technology

In this poster presented by Limula at Advanced Therapies 2025, see how the LimONE platform uses Akadeum’s microbubble-based CD3 selection to automate a full “one-pot” CAR-T manufacturing workflow. The workflow includes selection, activation, transduction, and expansion - all in a closed, end-to-end system. 

Key highlights:

• >93% CD3⁺ purity following automated microbubble-based selection
• Low exhaustion markers, comparable to gold-standard controls
• 19-fold T cell expansion in 7 days (>2 billion viable cells)
• Preservation of CD8+ naïve and Tcm memory phenotypes