--- title: "[Poster] Limula LimONE: all-in-one automated device for scalable cell therapy manufacturing" description: LimONE is a fully automated, closed platform for scalable CAR-T manufacturing, presented at ATC 2025 with integrated 7-day workflow data. --- ![Webinar Landing Page Banner-1](https://info.akadeum.com/hs-fs/hubfs/Webinar%20Landing%20Page%20Banner-1.png?width=2000&height=400&name=Webinar%20Landing%20Page%20Banner-1.png "Webinar Landing Page Banner-1") [![Akadeum Life Sciences, Inc](https://info.akadeum.com/hs-fs/hubfs/Akadeum-Logo-Transparent-2.png?width=796&height=797&name=Akadeum-Logo-Transparent-2.png "Akadeum Life Sciences, Inc")](https://www.akadeum.com/) ![Limula-colour-logo_540x305](https://info.akadeum.com/hs-fs/hubfs/Limula-colour-logo_540x305.jpg?width=156&height=88&name=Limula-colour-logo_540x305.jpg)     ![poster-tag](https://info.akadeum.com/hs-fs/hubfs/Akadeum_Poster-Tag_pink.png?width=100&name=Akadeum_Poster-Tag_pink.png) ## LimONE: all-in-one automated device for scalable cell therapy manufacturing At Advanced Therapies Congress 2025, Limula presented LimONE, a fully automated, closed platform integrating T-cell selection, activation, gene transfer, expansion, and harvest within a single vessel. Using Akadeum buoyancy-based CD3 negative selection integrated into LimONE, fresh PBMCs were processed and expanded to clinically relevant doses within a 7-day automated CAR-T workflow. Key Highlights: - >93% CD3+ purity with >68% recovery from fresh PBMCs, from input to seeding in 1 hour 45 minutes - Clinical-scale expansion from 120 million to 2.1 billion T cells in 7 days with >90% viability - Fully automated, closed end-to-end CAR-T manufacturing in a single consumable ** Download the poster** to review the CAR-T workflow and performance data **👉** ![Poster Screenshot_Limula_LimONE all-in-one automated device](https://info.akadeum.com/hs-fs/hubfs/Poster%20Screenshot_Limula_LimONE%20all-in-one%20automated%20device.png?width=1145&height=1627&name=Poster%20Screenshot_Limula_LimONE%20all-in-one%20automated%20device.png "Poster Screenshot_Limula_LimONE all-in-one automated device") --- ### Fill out the form to download the poster. 734.707.1233 info@akadeum.com 674 S Wagner Rd Ann Arbor, MI 48103 [![Akadeum Life Sciences](https://info.akadeum.com/hs-fs/hubfs/Akadeum_February2019%20/images/logo-footer.png?width=80&height=85&name=logo-footer.png)](https://www.akadeum.com/) - - - - [Products](https://www.akadeum.com/products/) - [Partnerships](https://www.akadeum.com/partnerships/) - [Solutions](https://www.akadeum.com/technology/) - [Resources](https://www.akadeum.com/resources/) © 2024 [Akadeum Life Sciences](https://www.akadeum.com/)