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title: [Poster] NIH + Akadeum SITC 2025
description: This collaborative study between the National Institutes of Health (NIH) and Akadeum Life Sciences demonstrates high-purity, high-viability T cell isolation using Akadeum’s GMP microbubble technology integrated with Cytiva’s Sepax™️ for a fully closed, automated workflow.
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## High-Purity Negative T Cell Isolation Using Akadeum’s GMP Microbubble Technology

This collaborative study between the National Institutes of Health (NIH) and Akadeum Life Sciences demonstrates high-purity, high-viability T cell isolation using Akadeum’s GMP microbubble technology integrated with Cytiva’s Sepax™️ for a fully closed, automated workflow.

Using a gentle, buoyancy-based negative selection approach, the process achieved >95% CD3⁺ purity and robust recovery in just 45 minutes. The resulting T cells remained untouched prior to activation, supporting the preservation of cell health, functionality, and phenotype critical to downstream CAR T manufacturing.<https://www.akadeum.com/product/microbubble-leukopak-human-t-cell-isolation-kit/>

![Poster Screenshot_NIH + Akadeum SITC 2025](https://info.akadeum.com/hs-fs/hubfs/Poster%20Screenshot_NIH%20+%20Akadeum%20SITC%202025.jpg?width=130&height=97&name=Poster%20Screenshot_NIH%20+%20Akadeum%20SITC%202025.jpg "Poster Screenshot_NIH + Akadeum SITC 2025")

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