More Potent CAR-T Cells via Akadeum Microbubbles Integrated with the Cytiva Sepax™ C-Pro

T cell isolation is a critical early step in CAR-T manufacturing, influencing cell recovery, phenotype, functional performance, and overall process robustness. As workflows move toward closed and scalable processing, integration with automated platforms becomes increasingly important.

In this webinar, we will present data from a fully closed T cell isolation workflow integrating Akadeum microbubbles with the Cytiva Sepax™ C-Pro Cell Processing System, compared directly to a positive selection magnetic isolation method in a CD19 CAR-T model.

Attendees will see how the Sepax/Akadeum workflow delivers higher T cell recovery, supports robust expansion and favorable phenotype, and shows functional performance in a CD19 CAR-T model, while maintaining comparable CD3+ purity.

Practical considerations for implementing Sepax-integrated isolation workflows in closed systems will also be discussed.

What you'll learn:

• How Akadeum microbubbles are integrated into a fully closed Sepax™ C-Pro cell processing workflow
• Performance comparison versus magnetic CD4/CD8 positive selection
• Impact of isolation method on cell recovery, expansion, phenotype, and function
• Considerations for closed-system implementation and scale-up

Your Hosts:

	Jesse Chen Senior Research Scientist and Applications Specialist Akadeum Life Sciences
	Eu Han Lee Senior Field Application Scientist Cytiva